Methylene blue can stain the cellular components like nucleus, DNA or RNA in cytoplasm.
Use of methylene blue:
Methylene Blue is a cationic stain. It is a positively charged blue dye that binds to negatively charged cell components like the nucleus, DNA, or RNA found in the cytoplasm.
It is a widely used stain for several purposes, including identifying germs and determining the cellular makeup of both plant and animal cells.
The cell wall and membrane are examples of the exterior cell structures that can be stained by gram staining.
In a collection of bacterial colonies, this stain is used to distinguish between gram positive and gram negative cells.
Learn more about the Gram's staining with the help of the given link: